FastQCFastQC Report
Wed 25 May 2016
SRR1700119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10397941
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110150.021.637941
GTATCAA204950.020.1441481
ATCAACG245300.016.3792293
TCAACGC247050.016.235894
CAACGCA249950.016.0475145
AACGCAG255650.015.7337146
TATCAAC259500.015.63032152
GTACATG253750.014.2809571
TACCTGG50150.013.9507972
TACATGG250550.013.8003152
GTACCTG53000.013.6661491
ACGCAGA295850.013.5895927
GTATTAG31600.013.5377581
CGAACTA5000.013.49857629
ACATGGG249550.013.4589683
CGCAGAG302650.013.2470888
AGAGTAC287750.013.05001911
GAGTACT171600.012.98039812
GTATTAA36800.012.7934471
CGTATCG2401.7203456E-712.18592115
Produced by FastQC (version 0.11.5)