FastQCFastQC Report
Wed 25 May 2016
SRR1700118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9623695
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102350.10635208202254955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104200.021.2472111
GTATCAA196600.020.9334641
TCAACGC244400.016.4670934
ATCAACG244550.016.4569913
CAACGCA247300.016.2648935
TATCAAC254450.015.98467352
AACGCAG252650.015.9738996
TAGACCG1951.1326847E-814.9974195
GTACCTG51350.014.5039461
GTACATG247550.014.2839691
TACCTGG51050.014.013262
ACGCAGA292500.013.8228537
TACATGG246450.013.5369282
CGCAGAG297900.013.5345248
AGAGTAC285800.013.422711
GTATTAG29350.013.3434691
CGCGGTA4700.012.92330943
ACATGGG247850.012.9158733
GAGTACT173950.012.77939412
ATAATAC34850.012.4584023
Produced by FastQC (version 0.11.5)