FastQCFastQC Report
Wed 25 May 2016
SRR1700117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12413589
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132190.1064881397313863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136600.023.132221
GTATCAA255800.020.998091
ATCAACG310650.016.9235133
TCAACGC312200.016.8394954
CAACGCA317000.016.5845095
TATCAAC325400.016.3084852
AACGCAG326850.016.1743226
ACGCAGA375750.014.0357967
GTACATG298850.013.8605491
CGCAGAG381900.013.7920948
TACATGG294800.013.5524782
AGAGTAC368000.013.40237111
ACATGGG297550.012.8526343
CGGTCGT4800.012.65643810
GAGTACT232400.012.53783612
GCAGAGT422350.012.4179029
CAGAGTA413550.012.36144510
GTATTAG36900.012.2070111
TATACTG49100.012.0497345
ACGTCCG4500.011.99785743
Produced by FastQC (version 0.11.5)