FastQCFastQC Report
Wed 25 May 2016
SRR1700116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7545970
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87120.1154523540379832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69450.028.9387361
GTATCAA145750.022.5940071
ATCAACG183250.017.6084123
TCAACGC184450.017.5182694
CAACGCA187050.017.2988415
TATCAAC191800.017.0030652
AACGCAG192600.016.8347246
CTACGCT2101.07320375E-1016.0838474
ATAACGC4850.014.8568323
TACCGTC2801.8189894E-1214.4651967
ACGCAGA225200.014.3681337
GTACATG196400.014.3587071
CGCAGAG228650.014.1275368
TACATGG197450.014.0071092
GTATTAG19900.013.87540151
TATAACG4850.013.4667732
GTATTAT26050.013.4667491
GTATTAA28750.013.4616131
ACATGGG197100.013.3550613
TACCTGG36850.013.0181522
Produced by FastQC (version 0.11.5)