FastQCFastQC Report
Wed 25 May 2016
SRR1700115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7608402
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA114310.15024179847489658No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79180.10406915933201216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG48600.018.0766961
TACCTGG48400.017.8231642
ATAACGC4200.016.6205983
GTATCAA203850.016.272361
ACCTGGG54300.015.3853843
GGTATCA130400.015.1760051
GTACATG184950.014.3114561
TATACCG3850.014.0265426
TACATGG183450.013.898252
TATACTG25250.013.5555285
TCAACGC239950.013.5325224
ATCAACG239000.013.5297813
CAACGCA242500.013.3902235
TATAACG5900.013.361262
AACGCAG248100.013.2230256
TATCAAC247700.013.1938892
ACATGGG183950.013.184093
AGAGTAC229550.013.15072411
GAGTACT140400.012.80147112
GTATAGA23100.012.7425121
Produced by FastQC (version 0.11.5)