FastQCFastQC Report
Wed 25 May 2016
SRR1700114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7917333
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA133450.1685542341088849No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81840.10336814177198306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA230450.015.9909341
ATAACGC5500.014.7392683
TATAACG4600.014.6881882
GTACCTG44550.014.3792061
GGTATCA151250.014.3073321
TACCTGG43600.014.2569742
GTACATG215500.014.2537371
TACATGG213000.013.9149312
CCGGTCG3101.8189894E-1213.7871749
AGAGTAC245400.013.50241811
TATACCG4000.013.5001576
GAGTACT144350.013.44860912
ATCAACG270800.013.2881413
TCAACGC272000.013.2874694
ACATGGG214000.013.2268873
CAACGCA275450.013.1128685
GTATATA36700.013.0140541
TATCAAC278200.013.0095872
AGTACTT148400.012.9148413
AACGCAG281100.012.8790186
Produced by FastQC (version 0.11.5)