FastQCFastQC Report
Wed 25 May 2016
SRR1700113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7715455
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82440.1068504709054748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80450.022.6794341
GTATCAA153000.021.05411
GTACCTG49250.019.8564071
TACCTGG49450.019.3126412
TATAACG4550.018.3160742
ATCAACG188000.016.7571643
TCAACGC189400.016.6570764
CAACGCA192050.016.485865
TATCAAC195250.016.2309952
AACGCAG197650.016.084856
ACCTGGG58750.016.0600453
ATAACGC5200.016.0228213
GTACCGA2051.2114469E-915.4595681
TAACGCA5400.015.4293854
GTACATG171650.014.1903031
ACGCAGA225700.014.0465887
TACATGG169200.013.8577412
GTACACG5250.013.7979281
CGCAGAG230550.013.7672218
ACATGGG169300.013.3408833
Produced by FastQC (version 0.11.5)