FastQCFastQC Report
Wed 25 May 2016
SRR1700112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4991328
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA79240.15875534527083773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59660.11952730816327838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG62700.031.5230711
TACCTGG61750.031.1852112
ACCTGGG67300.027.9694843
CCTGGGG49600.023.1516384
TATAACG5000.021.1712932
ATAACGC4900.020.6780553
GTACCGG3850.019.9940151
TAACGCA4950.019.5594444
GTACACG3450.017.7184831
CTGGGGG25450.016.8981365
TACCGGG4850.015.7890762
GTATCAA139100.015.7554231
GTCTCGC6400.014.5039311
GGTATCA92350.014.48881
TACACGG3600.014.3894732
GTACCCG3400.013.9837041
TATCACG4100.013.7333262
GTACATG108600.013.655051
GTATTAG13450.013.298011
ACACCGT3400.013.2352116
Produced by FastQC (version 0.11.5)