FastQCFastQC Report
Wed 25 May 2016
SRR1700111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8847943
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110750.019.0968761
GTATCAA195550.018.9098261
ATCAACG232300.015.5890913
TCAACGC234250.015.4689344
CAACGCA238000.015.2441255
TATCAAC242800.015.0758572
AACGCAG243100.015.0305486
GTACATG234600.014.3722871
GTACCTG49600.014.2440041
TACATGG234200.013.8693362
TACCTGG49250.013.8126832
CGCGGTA3650.013.5577743
ACATGGG233450.013.42855553
ATAATAC34850.013.3121563
TCTATAC22500.013.2122073
AGAGTAC255400.013.18489111
ACGCAGA280700.012.9856467
CGCAGAG285000.012.7468618
GTATTAG25450.012.6345491
TAATACT38300.012.3482244