FastQCFastQC Report
Wed 25 May 2016
SRR1700110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5852375
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83530.1427283795040475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59030.10086503342660032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG61400.027.6136651
TACCTGG61300.026.826352
ATAACGC4600.025.4302253
ACCTGGG64950.024.799253
GGTATCA67650.021.917151
TAACGCA4750.021.7855684
TATAACG4750.021.7855682
GTATCAA132450.020.9702281
CCTGGGG47850.020.0277584
ATCAACG158400.017.1418133
TCAACGC159450.017.0994744
CGCGGTA2650.016.97808843
CAACGCA161800.016.8650235
AACGCAG168250.016.3923076
TATCAAC166900.016.322722
GTACACG5500.015.639811
GTATTAG17100.014.826291
GTACCCG4350.014.5706631
TTAACGG3250.014.53597935
ACGCAGA191550.014.3183527
Produced by FastQC (version 0.11.5)