FastQCFastQC Report
Wed 25 May 2016
SRR1700109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5454194
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78670.1442376270444359No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58240.10678021353842566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG51800.026.5248321
TACCTGG50050.026.5190452
GGTATCA61550.025.0445211
ACCTGGG55600.023.1840763
GTATCAA120300.021.0551411
CCTGGGG41100.018.6647624
ATAACGC4950.018.633253
GTACACG4650.017.5244031
TAACGCA4750.017.5233884
TCAACGC148350.016.5897454
ATCAACG148150.016.5665873
TATAACG4800.016.4035112
CAACGCA151050.016.3378855
AACGCAG156650.015.9692456
TATCAAC154600.015.9481772
ACGTCGC1451.8149076E-615.51464922
TACACGG4650.015.4812832
GTCTCGC6300.014.3718641
GTACCCG3950.014.3263851
ACGCAGA178000.014.0300997
Produced by FastQC (version 0.11.5)