FastQCFastQC Report
Wed 25 May 2016
SRR1700108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4884008
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA124260.2544221876786443No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63630.13028234187986587No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA52100.10667468194155293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50350.10309155922758521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG35800.024.964881
TACCTGG35850.024.2221152
TATAACG3200.023.9024772
ATAACGC3400.021.1731263
ACCTGGG41550.020.3036543
TAGACCG1002.051634E-517.9971585
TAACGCA3650.015.40852454
CCTGGGG33900.015.3957994
ACGTCCG1553.628902E-614.51383743
GCGTACG950.005384961614.208281521
CGCGGTA1801.126411E-613.74782943
TCTGTCG6400.013.7108788
GTATCAA172450.013.67161751
TCGATTA2501.4897523E-913.497869544
TAACGGC2359.064024E-913.4021436
ACAACGC2205.5260898E-813.2933563
GTACATG112250.013.1827741
CGATTAC2602.6775524E-912.97871945
GCGGTAC2104.5251E-712.85511344
GAGTACT114250.012.76156412
Produced by FastQC (version 0.11.5)