FastQCFastQC Report
Wed 25 May 2016
SRR1700107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8745832
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91010.10406099728419206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97200.023.1453041
GTATCAA184150.021.0168271
TACCTGG56100.018.9674382
GTACCTG59650.018.3264961
ATCAACG225150.016.7160783
TCAACGC227250.016.6111034
ACCTGGG63550.016.5314793
TAACGCA4650.016.4488814
CAACGCA229850.016.4232035
TATCAAC233900.016.196542
AACGCAG235700.016.0919386
ACGTCCG5150.015.72554443
TATAACG5300.014.4315652
ATAACGC6950.014.2422393
GTATTAG27800.014.1659981
GTACATG236550.014.0744851
ACGCAGA271900.013.9428237
CGCAGAG276000.013.7520068
TACATGG234800.013.5954852
AGAGTAC275550.013.27677511
Produced by FastQC (version 0.11.5)