FastQCFastQC Report
Wed 25 May 2016
SRR1700106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7037576
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA140740.1999836307273982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76750.10905743682199666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA212950.015.7386741
GGTATCA140800.013.9832231
GTACCTG34550.013.6240431
AGAGTAC223750.013.16363311
TATACTG24200.013.1072135
GTACATG166400.013.0831541
GAGTACT142750.013.08281412
ATCAACG253400.012.9525513
TCAACGC253750.012.943554
TACCTGG34550.012.8921032
CAACGCA255150.012.8637125
TACATGG166550.012.7642052
AACGCAG258050.012.710436
TATCAAC260900.012.6923012
ACATGGG167700.012.2608263
AGTACTT152400.012.12152813
GTATAAG17750.012.1120031
TAGACAG17650.011.9809085
GTATTAT26850.011.9683641
GTATTAG19750.011.6875581
Produced by FastQC (version 0.11.5)