FastQCFastQC Report
Wed 25 May 2016
SRR1700105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8525096
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA194200.22779802127741436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102410.12012767950061794No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA88160.10341232521017946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG54400.020.4689241
TACCTGG55550.019.6409572
TAACGCA4850.017.6257174
ACCTGGG60600.017.5958673
TATAACG5650.016.7226772
ATAACGC5550.016.6186523
GTATCAA271700.014.893841
GTATTAG26300.013.9408121
GTACATG200250.013.5624311
AGAGTAC271400.013.36408211
GAGTACT175950.013.29927612
TACATGG195000.013.2437892
GTATTAT32650.012.6851821
CCTGGGG48400.012.642374
TCGATTA3750.012.5977544
ACATGGG196350.012.5798783
AGTACTT184750.012.54401913
TCAACGC321650.012.3862544
ATCAACG321900.012.3556683
CGCGGTA2756.17365E-912.27053643
Produced by FastQC (version 0.11.5)