FastQCFastQC Report
Wed 25 May 2016
SRR1700104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6215859
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77100.1240375626281098No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA62280.10019532296340698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94350.018.4771941
GTATCAA156150.018.2110461
GTACCTG31700.015.85553551
TATAACG3700.015.2191882
ATAACGC3650.014.8056743
GTACATG162650.014.7689131
ATCAACG190500.014.6803493
TACCTGG32700.014.6718562
TCAACGC192100.014.5580784
CAACGCA194650.014.4020635
TATCAAC196400.014.3472622
AACGCAG196950.014.336896
TACATGG161700.014.31977752
TAACGCA4050.013.899364
ACATGGG161850.013.7452943
AGAGTAC204150.012.91381611
GTATTAG18350.012.4615631
ACGCAGA226300.012.4576827
ACCTGGG36800.012.2986673
CGCAGAG229900.012.2305918
Produced by FastQC (version 0.11.5)