FastQCFastQC Report
Wed 25 May 2016
SRR1700103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8446297
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88710.10502827452077519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96800.022.3331171
GTATCAA176250.020.4344231
TATAACG3650.016.0424082
TCAACGC221850.015.9135694
ATCAACG220600.015.9118833
CAACGCA225050.015.6772895
TATCAAC230050.015.3305812
AACGCAG233100.015.2216496
ATAACGC5100.015.0103133
GTACATG230400.014.5805111
CGCGGTA3900.014.41940843
GTACCTG42250.014.412751
TATCCCG3850.014.0356165
TACATGG231850.014.0167812
ACATGGG234550.013.3624113
ACGCAGA266400.013.3363887
GTATTAG24500.013.3050861
CGCAGAG270350.013.1301788
TACCTGG44550.013.0425072
AGAGTAC261950.012.76975411
Produced by FastQC (version 0.11.5)