FastQCFastQC Report
Wed 25 May 2016
SRR1700102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8792129
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97950.11140646366767366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94650.022.7821141
GTATCAA182600.021.881421
TCAACGC224550.017.4401284
ATCAACG224250.017.4333323
CAACGCA227350.017.245155
AACGCAG233400.016.7929636
TATCAAC235150.016.7536452
ACGCAGA268350.014.5810217
CGCAGAG273750.014.2976288
AGAGTAC268700.013.80453811
GTACATG236900.013.7968181
GAGTACT156900.013.69144612
TACATGG239050.013.323712
CAGAGTA295800.012.94286110
GCAGAGT300700.012.9414099
GTATTAA30950.012.8804111
ACATGGG237950.012.8143363
AGTACTT165650.012.79170213
GTATACG3900.012.7772031
ATAATAC31200.012.6312583
Produced by FastQC (version 0.11.5)