FastQCFastQC Report
Wed 25 May 2016
SRR1700101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6675753
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74170.11110357138737756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57250.027.2071421
GTATCAA122400.022.8061331
ATCAACG149900.018.145883
TCAACGC150700.018.0794324
CAACGCA154100.017.6951485
TATCAAC158300.017.3423182
AACGCAG157500.017.3284996
ACGCAGA185050.014.73693757
GTACATG171150.014.68304351
TAGACCG1402.2171753E-514.4752885
CGCAGAG189750.014.3801088
TACATGG169650.014.1642932
ACATGGG170400.013.742843
GTACCTG35650.013.717161
CTACCCG3350.013.42976210
TACACCG3900.013.2793245
TACCTGG36000.013.1997072
AGAGTAC201450.013.06476811
GCAGAGT211650.012.8709019
CAGAGTA211600.012.72511210
Produced by FastQC (version 0.11.5)