FastQCFastQC Report
Wed 25 May 2016
SRR1700100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6601601
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67700.022.4427241
GTATCAA123850.020.6048581
GTACCTG38600.018.3611031
TACCTGG38450.017.9824582
TCAACGC152000.016.4291824
ATCAACG152350.016.3027553
CAACGCA154800.016.1901995
AACGCAG158900.015.7885856
ACCTGGG43100.015.6737173
TATCAAC161200.015.6340692
GTACATG162200.014.6302651
GTATTAG17850.014.334481
ATAACGC3950.014.2518383
TACATGG162600.013.8927232
ACGCAGA183350.013.63458257
CGCGGTA2855.4569682E-1113.4173943
TATACTG23850.013.4068865
ACGAACG1851.5354944E-613.37472729
CGCAGAG188350.013.2448268
ACATGGG163550.013.148643
Produced by FastQC (version 0.11.5)