FastQCFastQC Report
Wed 25 May 2016
SRR1700099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5531476
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA93680.16935805199190956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70010.12656658005928256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG37500.021.9788951
TACCTGG37600.021.5635972
TATAACG3050.018.4606382
ACCTGGG42850.018.4440693
TAACGCA3100.018.158454
GTATCAA161250.016.1485611
ATAACGC4100.015.9262893
TACACGG4300.015.1892422
GTACATG135400.014.2480911
GTATTAG16700.013.8299131
TACATGG134650.013.7657552
ATCAACG190000.013.4624823
AGAGTAC175350.013.43102911
ACATGGG134200.013.422643
TCAACGC191100.013.4085564
GTACACG5100.013.3194671
CAACGCA192350.013.2745955
AACGCAG194550.013.1726986
TTAACGG3101.6370905E-1113.06120235
CCTGGGG35450.013.0208124
Produced by FastQC (version 0.11.5)