FastQCFastQC Report
Wed 25 May 2016
SRR1700098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4259515
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68930.1618259355818679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51230.12027190889103573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49600.024.228011
GTATCAA95600.021.47111
TACCGCG1452.2009772E-1020.1731878
GTACCTG27000.018.021031
TACCTGG25850.017.666192
ATCAACG119650.016.733423
TCAACGC120150.016.7012314
CAACGCA122200.016.4210555
ACCTGGG26650.016.2073273
TATCAAC125450.016.1032332
AACGCAG126200.015.9005786
TAACGCA2950.014.489024
GCGTAGA1108.2253845E-414.4015851
ACGCAGA144300.013.8622787
CGCAGAG146400.013.7095428
GTACATG102300.013.5388171
TCTATAC10750.013.3930313
GTATAGA13350.013.3921621
GCCGTTA2205.5248165E-813.293467515
AGAGTAC142550.013.2751111
Produced by FastQC (version 0.11.5)