FastQCFastQC Report
Wed 25 May 2016
SRR1700097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5795887
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96270.16610054681880446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70180.1210858665809047No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA63400.10938791594798172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA157700.018.623011
GGTATCA94200.017.365781
ATCAACG198800.014.5750983
TCAACGC199700.014.5094124
TATCAAC203200.014.3259222
CAACGCA202900.014.30275255
AACGCAG205250.014.1610376
GTACATG149250.013.689271
GAGTACT135650.013.45180212
GTATTAG17450.013.3551471
AGAGTAC205500.013.2590311
TACATGG147750.013.2009022
AGTACTT141350.012.90924213
TTACCGG1751.2696655E-512.85705210
GTACGAC2104.5258275E-712.8550563
TACGACC2857.767085E-1012.6295294
ACATGGG148100.012.5621063
ATAACGC3601.8189894E-1212.4979713
TTATACT25800.012.4689054
TATACTG21750.012.41177755
Produced by FastQC (version 0.11.5)