FastQCFastQC Report
Wed 25 May 2016
SRR1700096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4649916
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75190.1617018457967843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56720.121980698145945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61400.023.6257571
GTATCAA108950.019.3383121
ATCAACG137550.015.0791293
TCAACGC137800.015.0680974
CCGATCG1056.0439325E-414.999299
CAACGCA139600.014.873815
TATCAAC141950.014.6751152
AACGCAG142600.014.5924476
ACGTCCG1859.6539225E-814.59202143
CGCGCAT1000.007561150513.49849134
GTACATG121550.013.3679731
GTATTAG13100.013.3019571
TACATGG122950.012.7712332
CGCCGTA1952.7689857E-612.69006925
CGCACGA1607.831547E-512.65633112
ACGCAGA164400.012.6317027
TAGACCG1250.002256289112.5977785
AGAGTAC158350.012.41877311
GTACCTG20800.012.4033111
GTATTAT16550.012.3066721
Produced by FastQC (version 0.11.5)