FastQCFastQC Report
Wed 25 May 2016
SRR1700095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2116169
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38030.17971154477737838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26590.1256515902085325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27600.022.6345181
GTATCAA53450.020.072521
CTAACCG1053.1185373E-517.1402221
CGTATCG952.8235643E-416.57678815
TAACGGC952.824073E-416.57639736
ATCAACG63850.016.3130743
TCAACGC64000.016.274844
CAACGCA64850.016.0615235
AACGCAG66700.015.6834956
TATCAAC67550.015.6193582
TCCCGTA1056.042553E-414.9991112
CGGTACC1251.3831288E-414.39778445
GAGTACT51200.014.36926612
AGAGTAC72600.013.91446411
ACGCAGA75400.013.8450047
CGCAGAG75800.013.8016258
AGTACTT53050.013.74093813
GTACATG50750.013.5138111
GTATTAG7150.013.29581
TACATGG50200.013.2200782
Produced by FastQC (version 0.11.5)