FastQCFastQC Report
Wed 25 May 2016
SRR1700094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6160055
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85880.13941433964469474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61990.10063221838116705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74450.022.6776051
GTATCAA145250.021.6270071
TCAACGC181250.017.0537134
ATCAACG181400.017.039613
CAACGCA184400.016.8111925
TATCAAC186800.016.6433732
AACGCAG188050.016.5447046
TAGACCG1351.5862715E-514.9975025
ACGCAGA218400.014.2371167
CGCAGAG220900.014.06580458
AGAGTAC213700.013.92951611
GAGTACT135450.013.67104112
GTACATG167700.013.4955331
GTACCTG29300.013.0539571
GTATTAG17700.013.0421741
AGTACTT139150.013.01647513
TACATGG166350.013.0095552
CAGAGTA239450.012.83561210
GCAGAGT241800.012.8221119
ACATGGG167500.012.3830133
Produced by FastQC (version 0.11.5)