FastQCFastQC Report
Wed 25 May 2016
SRR1700093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367620
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56750.2396921803329926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41910.1770132031322594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31170.13165119402606837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34900.024.2489381
CGGTATA602.4718142E-422.495652
GTATCAA65100.021.1333891
TATACCG654.2455955E-420.7652155
ATAACGC1507.2941475E-917.996523
GCGGTAT850.00247090615.9727191
ATCAACG83800.015.9724473
TCAACGC84500.015.7602654
TATCAAC84950.015.7297432
CAACGCA85350.015.6560235
AACGCAG87250.015.3150896
ACCGAAC900.003755041415.0002678
CGTTATA900.00376032114.9970992
TATAACG1705.9081685E-714.5560082
GTGTACG1301.8368923E-413.9249351
ACGCAGA98600.013.5550087
TACCTGG10650.013.5185132
CTAGGAC4350.013.4456763
CGCAGAG99750.013.3987358
AGAGTAC94500.013.38175611
Produced by FastQC (version 0.11.5)