FastQCFastQC Report
Wed 25 May 2016
SRR1700092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3850761
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62180.1614745760643156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47910.12441696589323512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51600.022.0145971
GTATCAA92850.020.0086571
TACCGTC1202.4962173E-718.7530217
ATACCGT2450.018.3688746
GTACCTG18850.018.0068021
ACCGTCG850.002569436815.8797548
ATCAACG116150.015.5644943
TCAACGC116850.015.5097884
TACCTGG20800.015.4816992
CAACGCA118250.015.3452015
GTATTAG10600.015.1568571
TATCAAC120250.015.1498852
AACGCAG120650.015.0323316
ACCGTCC1209.7752505E-514.9975458
CGCCGTA900.003762096614.99637625
ATAACGC2407.9853635E-1014.0707963
TATACTG12950.013.5601195
GTACATG93700.013.2100491
ACGCAGA138100.013.0687247
AGAGTAC132450.013.06128311
Produced by FastQC (version 0.11.5)