FastQCFastQC Report
Wed 25 May 2016
SRR1700091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6257435
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77040.12311753937515932No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA65450.10459557310623283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94200.018.7497751
GTATCAA160250.018.6521221
CGGTCGA952.826443E-416.57542620
ATAACGC3700.015.8230053
TCAACGC195150.014.93079954
ATCAACG195350.014.9039873
CAACGCA198100.014.7425585
TATCAAC200600.014.6176412
AACGCAG202600.014.45922856
GTACATG157700.014.2009361
GTATTAG18200.014.1835791
CGTTATA1656.8531954E-613.6493922
TACATGG157350.013.6402842
GTATTAT20650.013.5973421
GTACCTG32350.013.5793761
AGAGTAC202050.013.47109511
GAGTACT127250.013.47018512
GTATTAA20950.013.0783741
ACATGGG159000.012.98642353
TACCTGG31150.012.8694282
Produced by FastQC (version 0.11.5)