FastQCFastQC Report
Wed 25 May 2016
SRR1700090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5133254
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68540.13352154403425195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52380.10204053802909423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78650.019.8786051
GTATCAA128400.017.8857691
TAGACCG1104.620403E-516.3727785
CGATCGC1251.3846421E-414.39667715
TATAACG2853.6379788E-1214.2213772
ATCAACG162500.013.853893
TCAACGC163800.013.8264034
TATCAAC166200.013.724372
CAACGCA165900.013.6242435
CGCGGTA2002.502711E-713.49688443
AACGCAG169050.013.4433346
TACACCG3001.2732926E-1012.7571245
GTACATG139450.012.5744731
TACATGG136900.012.4839582
GTATTAG13650.012.4318341
CTACACT11600.012.032584
TAGACTG11100.011.9661415
CCGGTCG2654.856338E-811.8842819
CGGTCGT2654.856338E-811.88428110
ACATGGG138800.011.8726233
Produced by FastQC (version 0.11.5)