FastQCFastQC Report
Wed 25 May 2016
SRR1700089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6632571
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90780.13687000109007502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72650.027.4020961
GTATCAA150500.022.5878411
ATCAACG186750.017.8595093
TCAACGC187900.017.83414
CAACGCA192200.017.4702575
TATCAAC194150.017.3225652
AACGCAG195750.017.1719996
GCGTACG1654.219437E-714.99658621
ACGCAGA225850.014.883757
CGCAGAG230200.014.5986448
GTACATG188350.014.3692071
TACATGG184550.014.1224182
TAGACCG1453.082402E-513.9781465
GTATTAG21500.013.904841
AGAGTAC235400.013.79837611
CGATCGG1000.007570414313.49611243
ACATGGG187550.013.3885293
CAGAGTA248900.013.32109210
GCAGAGT252800.013.2757489
GAGTACT145200.013.15250112
Produced by FastQC (version 0.11.5)