FastQCFastQC Report
Wed 25 May 2016
SRR1700088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3849905
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49520.1286265505252727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53650.019.402071
GTATCAA92800.018.483371
TATAACG2100.018.231782
TAACGCA2400.017.8238164
ATAACGC2950.017.5535163
ACACCGT2800.015.2682276
TCAACGC114800.014.7284264
ATCAACG114250.014.6810923
CAACGCA116800.014.553335
CGTGCGA1251.3840971E-414.39716910
TATCAAC118900.014.300942
AACGCAG119300.014.2585186
TAACGGC1951.838198E-713.84271336
TATACTG11750.013.7959915
GTACCTG19550.013.5425041
TAGACCG1000.00752358813.50857455
CGATTAC2501.4915713E-913.49664645
GTATTAG12250.013.4848661
GTACATG104350.013.2931511
GTATTAA11600.013.0700351
Produced by FastQC (version 0.11.5)