FastQCFastQC Report
Wed 25 May 2016
SRR1700087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5230007
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71670.1370361454583139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55950.026.3333841
GTATCAA108850.022.1227421
TCAACGC135800.017.4915584
ATCAACG135650.017.444513
CAACGCA137750.017.2439465
TATCAAC139250.017.1263122
AACGCAG142400.016.7501346
ACGCAGA163200.014.5608587
CGCAGAG166700.014.1962558
GTATTAT17500.014.0965951
GTACATG136500.014.0932791
GGGACCG3850.014.0354495
GGACCGT3050.014.0176796
TACATGG137000.013.5940252
TATACTG20100.013.4419115
AGAGTAC168650.013.36518711
GAGTACT106000.013.28501712
ACATGGG135850.013.0599763
GCAGAGT182400.012.9496529
GTATTAG15850.012.8510621
Produced by FastQC (version 0.11.5)