FastQCFastQC Report
Wed 25 May 2016
SRR1700086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9631414
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA204320.21213915215356746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123520.12824700506073147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG3950.020.975826
AACGCGG5250.015.7818077
TACCTGG52050.015.144322
GTATCAA318200.014.5474891
GTACCTG57400.014.44267751
ACGCGGA6050.014.4352258
ACGATCG2102.125853E-814.29759520
CGCATAG3950.014.1728528
TACCGTC3201.8189894E-1213.2959067
AGAGTAC292800.013.1996911
ACCTGGG58650.013.1346433
GAGTACT187850.013.05259712
GTACATG230100.012.9896391
AGTACTT196850.012.94489813
TACATGG227450.012.4448292
TATACTG32800.012.3573635
TCAACGC370700.012.2153714
TATAACG4600.012.1705452
ATCAACG371850.012.15895753
CAACGCA373700.012.1285385
Produced by FastQC (version 0.11.5)