FastQCFastQC Report
Wed 25 May 2016
SRR1700085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5817516
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA156370.26879169734986547No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA69490.1194496070144027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG50550.024.7048112
GTACCTG51200.024.6813851
ACCTGGG56050.021.196693
TCGACGC1503.45608E-1019.501223
CCTGGGG46950.015.2869214
CCGGTCG2850.014.9990069
TAACGCA4300.014.6505714
CGACGCA2254.9149094E-913.9994334
GTACATG131700.013.9737471
GTACCGG4200.013.94442751
AGAGTAC163950.013.91490611
GTACACG4700.013.8987841
ATACCGC3400.013.8961377
ATAACGC4300.013.6055043
GTATCAA199850.013.3451711
TACATGG131250.013.3379792
GAGTACT102300.013.32756212
ACGTCCG2257.400922E-812.99992143
CGGCTAT4050.012.7771530
ACATGGG136050.012.7185473
Produced by FastQC (version 0.11.5)