FastQCFastQC Report
Wed 25 May 2016
SRR1700084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9025881
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA194050.020.5821551
GGTATCA106950.020.178111
ATCAACG237850.016.4238593
TCAACGC239500.016.2989654
TATCAAC245950.015.9561592
CAACGCA244900.015.8201465
AACGCAG251550.015.4460446
GTACATG239500.014.0708971
CGATCGT4250.013.7638841
TACATGG240850.013.6219592
GAGTACT151950.013.53309512
AGAGTAC266900.013.43666311
ACGCAGA289050.013.4033047
CGCAGAG294000.013.1317218
AGTACTT157100.013.10377913
TCGCGAT5200.012.98013438
ACATGGG244100.012.9335743
TAGACCG2104.519934E-712.8566575
TTTCGCG4900.012.85644336
GTATTAT23100.012.677311
Produced by FastQC (version 0.11.5)