FastQCFastQC Report
Wed 25 May 2016
SRR1700083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14212121
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN151710.1067469099088025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG4652.3790017E-719.44003745
GTATCAA315550.018.0013521
GGTATCA185750.017.255711
ACGACCG4000.001141141915.06602845
TCAACGC374400.014.7852034
ATCAACG376200.014.7437483
CAACGCA382800.014.4492485
TATCAAC391600.014.2944732
AACGCAG392650.014.1510346
AACGCGA6600.001990782613.69638945
GTACATG351000.013.3236991
AGAGTAC391650.012.97208911
GTATTAG39250.012.9265981
GAGTACT216900.012.91025412
TACATGG352200.012.8525172
CGCGTAT1200.001942278912.85184712
TATACTG45500.012.3985825
ACGCAGA446150.012.3800447
CGCAGAG453700.012.1254688
AGTACTT230050.012.05736113
Produced by FastQC (version 0.11.5)