FastQCFastQC Report
Wed 25 May 2016
SRR1700082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12849566
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN130010.10117851451169635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116550.023.7900891
GTATCAA228650.022.49731
AACGCGA7651.6204564E-519.65738745
TCAACGC288450.017.3427964
ATCAACG289800.017.284823
CAACGCA298100.016.7739895
TATCAAC302400.016.747722
AACGCAG307150.016.2901086
ACGCAGA358050.013.9435567
GTACATG372900.013.7886881
TACACCG7900.013.6688765
CGCAGAG367050.013.5416368
TACATGG377100.013.3074232
AGAGTAC366000.012.90025811
GAGTACT178600.012.89116412
CTATACT23450.012.59294
CAGAGTA389300.012.45075210
ACATGGG388000.012.4330553
CGCGCAA8650.012.225944518
GCAGAGT407750.012.1143189
Produced by FastQC (version 0.11.5)