FastQCFastQC Report
Wed 25 May 2016
SRR1700081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11281966
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN117810.10442328934513719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA252050.016.7512251
TTATACG5450.00605873616.6087645
GGTATCA157050.015.3523031
CGCGCAA4950.014.68787118
ACGATCG2551.8553692E-1013.824491520
GTACATG303150.013.7529391
ATCAACG301900.013.5627053
TCAACGC305350.013.42384054
CAACGCA309000.013.2581435
TATCAAC314150.013.1960032
TACATGG303300.013.137622
AACGCAG315800.012.9902556
ATCGCGA3400.012.96046223
GTATTAG27950.012.7857011
AATCGCG5750.012.64434115
GTATTAT28100.012.6389461
TATACTG33300.012.5077545
ACATGGG310550.012.4398193
CGAACTA6950.012.36585829
AGAGTAC301650.012.1461611
Produced by FastQC (version 0.11.5)