FastQCFastQC Report
Wed 25 May 2016
SRR1700080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8226222
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56000.024.978751
GTATCAA113200.022.3139671
CGCGTAA1001.7919017E-518.28278510
TCAACGC138950.017.7602184
ATCAACG139550.017.7005793
CAACGCA143450.017.1874455
TATCAAC146550.016.9622692
AACGCAG147250.016.3893226
GTACATG220900.014.84995651
GTACCTG51600.014.7944861
TACCTGG49750.014.6561622
TACATGG223850.014.081512
TAGACCG2601.891749E-1013.8057755
ACGCAGA173000.013.8045597
ACATGGG229500.013.4904483
CGCAGAG177950.013.3306628
GTATTAA21200.012.7091371
GCGCGTT4500.012.46339531
GTATTAG21850.012.4338221
ACCTGGG55950.012.3904873
Produced by FastQC (version 0.11.5)