FastQCFastQC Report
Wed 25 May 2016
SRR1700079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7658563
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90900.11869067343312316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86050.023.7451021
ATACGCG500.00230435422.4347085
GTATCAA174400.021.9513591
TCAACGC215500.017.3231334
ATCAACG216250.017.3053423
CAACGCA218800.017.0208475
TATCAAC222650.016.9086722
CAACGCG3300.016.6217546
AACGCAG222650.016.5368336
CGCGGTA3250.015.18607543
ACGCAGA252100.014.4599697
AACGCGG4300.014.3507577
CGCAGAG256350.014.2113238
GTACATG211950.013.8566361
GAGTACT150500.013.80406112
AGAGTAC252200.013.72019611
AGTACTT158100.013.26723113
TAACGGC4100.013.13225536
TACATGG210000.013.1302442
GCAGAGT280400.012.9435089
Produced by FastQC (version 0.11.5)