FastQCFastQC Report
Wed 25 May 2016
SRR1700078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9414127
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99120.10528857322617381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98100.023.9391651
GTATCAA201150.022.7250081
CAACGCG3250.018.987236
ATCAACG244950.018.2079013
TCAACGC245400.018.14674
CAACGCA250750.017.7505765
TATCAAC252600.017.6920952
AACGCAG254450.017.2097386
GTACCTG56950.015.572191
ACGCAGA292150.014.7855397
TACCTGG58650.014.6123012
CGCAGAG296500.014.5377858
AACGCGG4400.014.0246597
GTACATG236550.013.9901021
AGAGTAC293800.013.95570711
GAGTACT182100.013.86862912
TACATGG233650.013.5866982
ACCTGGG62100.013.2585323
GCAGAGT325800.013.2023049
CAGAGTA321150.013.18708210
Produced by FastQC (version 0.11.5)