FastQCFastQC Report
Wed 25 May 2016
SRR1700077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11512088
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA448300.3894167591491656No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA196380.17058590935024126No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT148010.12856920482192283No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT138500.12030832286897043No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC131610.11432330955079566No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124710.10832960971111408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG4200.018.1280526
TACCTGG69400.014.52142
GTACCTG72300.014.4423231
ACGCGGA5700.013.3575118
GAGTACT213600.013.10416912
GTATTAG33400.013.0821041
AGAGTAC360550.012.96778411
ACCTGGG73750.012.9664563
GTACATG292600.012.6510091
AGTACTT224650.012.60906713
TACATGG293000.012.2906232
AACGCGG6200.012.28029257
TTTCGCG4150.011.8712436
GTACTTT237200.011.82865214
GTATATA45500.011.7699851
GTATTAA38650.011.5949771
CGATCGT4250.011.59191741
ACATGGG301150.011.5861733
CGTTCCG4650.011.55762234
ATACCGC7600.011.4913887
Produced by FastQC (version 0.11.5)