FastQCFastQC Report
Wed 25 May 2016
SRR1700076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12335276
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA278500.018.7145541
GTACCTG81050.018.3020571
TACCTGG83550.017.0203572
GGTATCA163850.016.287721
ATCAACG328900.015.4766483
TCAACGC329650.015.4559644
CAACGCG4500.015.4266336
ACCTGGG89650.015.3126933
CAACGCA335450.015.1873725
TATCAAC347750.014.7665242
AACGCAG340600.014.5827336
TATAACG7050.014.2943622
GTACATG284050.013.2370791
AGAGTAC339200.013.11049211
GAGTACT200350.012.92004712
ACGCAGA384900.012.8287017
TACATGG283850.012.8205272
TAACGCA7200.012.75324
CGCAGAG392000.012.5792068
AGTACTT212600.012.533648513
Produced by FastQC (version 0.11.5)