FastQCFastQC Report
Wed 25 May 2016
SRR1700075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8262915
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA182760.22118102388805888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA3650.014.79472418
GTACATG215100.013.9902421
GTACCTG49800.013.7047921
TACATGG215150.013.53391552
GTATCAA265650.013.4224021
TACCTGG48250.013.198352
CGCAACG4400.012.78473120
ACATGGG219550.012.7194663
AGAGTAC233950.012.70373511
GTATTAG18100.012.6934811
GAGTACT129700.012.33335712
TTTCGCG2951.3387762E-912.2025239
GTATTAA26150.012.1452541
TATACTG27950.012.0750075
CAACGCG3001.7425918E-911.9998745
ATCGCGC4500.011.99907616
ACCTGGG51550.011.9605853
GTATATA31300.011.9460121
CATGGGG148300.011.9250264
TACCGTC4200.011.7848787
Produced by FastQC (version 0.11.5)