FastQCFastQC Report
Wed 25 May 2016
SRR1700074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12063489
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126330.10472094764624065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA281950.019.088571
GGTATCA162850.018.4094491
CAACGCG4550.016.7334736
ATCAACG340400.015.5063073
TCAACGC340850.015.4864124
CAACGCA348150.015.1477615
TATCAAC352600.015.0841082
AACGCAG353950.014.5197566
GTACATG294450.013.5450581
AACGCGG5650.013.4756297
ACGCGGA6050.013.3249538
GAGTACT213150.013.1635112
TACATGG292750.013.1191472
ATCGCGA3008.367351E-1113.0866123
AGAGTAC359150.013.0184911
ACGCAGA402250.012.7651687
TATACTG38700.012.7302135
AGTACTT225550.012.68791813
CGCAGAG408250.012.5117398
GTATTAG34000.012.4553321
Produced by FastQC (version 0.11.5)