FastQCFastQC Report
Wed 25 May 2016
SRR1700073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595603
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29510.18494575405034963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18820.11794913897755269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG11750.024.5110842
ACCTGGG11350.024.38373
TATAACG1152.6557245E-1023.4786872
GTACCTG12950.023.307111
TCAACGG754.607877E-520.997755
GTATCAA39600.019.45291
TAACGCA1202.500983E-718.747994
ATAACGC1354.2744432E-818.3336663
GTACCGG1002.028435E-518.0195271
CCTGGGG9200.017.117734
GGTATCA22450.016.3540251
TCAACGC45900.016.1747364
ATCAACG46650.015.8684553
CAACGCA46500.015.8208835
AACGCAG48650.015.5379016
TATCAAC48600.015.4169452
ATTATAC5600.015.2681333
GTATAAC3600.015.0162731
TCGTATG2850.014.99886140
TAGTACT4400.014.8279544
Produced by FastQC (version 0.11.5)