FastQCFastQC Report
Wed 25 May 2016
SRR1700072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7017895
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA250570.357044384391616No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA104670.14914728704262462No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT78850.11235562800526369No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT76370.10882180482894087No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC73800.10515973806960635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG50200.019.3395081
TACCTGG48350.019.1743452
ACCTGGG54150.016.5388153
CCGAACG1104.6463312E-516.36224213
TATAACG4700.016.2779922
ATAACGC6000.016.1263833
TAACGCA4350.016.0336884
CAACGCG3700.015.201955
GTACATG170150.014.8139211
CGCGGTA3250.014.538259543
ATCGCGA1553.6181464E-614.51820323
AACGCGG4450.014.1559966
TACATGG172000.014.1552842
TATACTG22250.014.0554845
ACGATCG1453.1069154E-513.96641820
ACCGTCC2652.4556357E-1013.583758
CGGACGT3009.094947E-1213.49894636
GAGTACT127550.013.44064212
ACATGGG173150.013.3725023
AGAGTAC206500.013.32453111
Produced by FastQC (version 0.11.5)