FastQCFastQC Report
Wed 25 May 2016
SRR1700071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12669454
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN162750.12845857445790482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT1550.001013886919.45320145
GTATCAA316350.015.2098971
GTACCTG79550.015.0313341
TACCTGG79450.014.5918392
GTACATG345000.013.1602671
GCGCGTT4600.013.10976745
GTATTAG34100.012.9393981
ATCAACG368150.012.7027463
TCAACGC368650.012.6974244
GTATATA49300.012.66420651
TACATGG345100.012.645572
CAACGCA372400.012.5695625
ACCTGGG89350.012.4558643
AGAGTAC335850.012.44459411
TATCAAC382900.012.3699872
AACGCAG380700.012.3558826
TATACTG41050.012.3478115
GAGTACT155750.012.27864512
GGTATCA210650.012.0964791
ACATGGG352400.012.0072163
Produced by FastQC (version 0.11.5)