FastQCFastQC Report
Wed 25 May 2016
SRR1700070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8196653
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN234100.2856043802269048No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN108830.1327737065360703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN99700.12163501370620422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG80250.024.382811
TACCTGG77950.024.3161682
ACCTGGG83000.022.3569363
TAGGCCG4752.2501745E-518.97722645
CCTGGGG69650.016.7065124
TACACGG7350.014.99323752
ATAACGC5800.014.8188643
GTACACG7050.014.70665551
TATAACG5750.014.5656052
GTACATG190750.013.8200161
CGATCGT2103.915011E-813.6480141
TACATGG188700.013.2801012
CGACGCT2502.0354491E-913.21943821
TTAACGG2901.03682396E-1012.916409535
TACGTCG2350.003275608212.78607545
GTATAGA20450.012.6211151
ACATGGG194200.012.5171253
AGAGTAC232250.012.426784511
GAGTACT132200.012.1989612
TAACGGC3154.110916E-1011.8913736
Produced by FastQC (version 0.11.5)